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| Soufi-kechaou, Emna. |
| This work is conducted in the framework of cuttlefish Sepia officinalis by-products up-grading from conditioning industries. It concerns the implementation of the enzymatic hydrolysis and the membrane separation processes to obtain valuable compounds such as peptides and lipids. The techniques used in this study belong to "clean technologies", environmentally sound involving moderate investment and low energy consumption. Hydrolysis of cuttlefish viscera had been carried out in two steps. The first one had the objective to determine the efficiency of the enzymes on the matrixes investigated as well as the study area. Once the enzyme had been chosen, the second step was to optimize enzymatic hydrolysis using experimental designs, in order to obtain the... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00055/16645/14200.pdf |
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| Soufi-kechaou, Emna; Jaouen, Pascal; Ben Amar, Raja; Berge, Jean-pascal. |
| Enzymatic hydrolysis of cuttlefish viscera by Protamex, Pepsin, Delvolase and Multifect Neutral was investigated in a batch reactor during 6 hours with an hourly sampling. The influence of the enzyme used and the hydrolysis conditions (operating pH, temperature) was studied with regards to the extent of proteolytic degradation and to molecular weight distribution of the peptides. After 360 min of hydrolysis, cuttlefish viscera treated with Pepsin yielded significantly (p < 0.05) higher protein recoveries compared to the substrate autolysed or treated with other external proteases. However, the amino acid composition showed that this was Protamex hydrolysis which gave the highest percentage of essential amino acids. At least, according to the FPLC... |
| Tipo: Text |
Palavras-chave: Enzymatic hydrolysis; Cuttlefish Protein Hydrolysates (CPH); Cuttlefish By-Products (CBP); Essential Amino Acids (EAA); Protein recovery. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00114/22508/20191.pdf |
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